Phylogenetic characterization of the energy taxis receptor Aer in Pseudomonas and phenotypic characterization in Pseudomonas pseudoalcaligenes KF707
| Autor: | Sean C. Booth, Raymond J. Turner |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Genetics
0303 health sciences animal structures endocrine system diseases biology Phylogenetic tree 030306 microbiology Sequence analysis Energy taxis Pseudomonas urologic and male genital diseases biology.organism_classification Microbiology Pseudomonas putida 03 medical and health sciences Pseudomonas pseudoalcaligenes Phylogenetics Horizontal gene transfer human activities 030304 developmental biology |
| Zdroj: | Microbiology. 165:1331-1344 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.000864 |
| Popis: | Chemotaxis allows bacteria to sense gradients in their environment and respond by directing their swimming. Aer is a receptor that, instead of responding to a specific chemoattractant, allows bacteria to sense cellular energy levels and move towards favourable environments. In Pseudomonas, the number of apparent Aer homologues differs between the only two species it has been characterized in, Pseudomonas aeruginosa and Pseudomonas putida. Here we combined bioinformatic approaches with deletional mutagenesis in Pseudomonas pseudoalcaligenes KF707 to further characterize Aer. It was determined that the number of Aer homologues varies between zero and four throughout the genus Pseudomonas, and they were phylogenetically classified into five subgroups. We also used sequence analysis to show that these homologous receptors differ in their HAMP signal transduction domains. Genetic analysis also indicated that some Aer homologues have likely been subject to horizontal transfer. P. pseudoalcaligenes KF707 was unique among strains for having three Aer homologues as well as the receptors CttP and McpB. Phenotypic characterization in this strain showed that the most prevalent homologue of Aer was key, but not essential, for energy taxis. This study demonstrates that energy taxis in Pseudomonas varies between species and provides a new naming convention and associated phylogenetic details for Aer chemoreceptors. |
| Databáze: | OpenAIRE |
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