Fabrication of branched polyethylenimin/alginic acid/poly(cyclohexane-1,4-diyl acetone dimethylene ketal as a nano size carrier for controlled release of 5-fluorouracil
| Autor: | Chen-Yu Kao, Doris Yii, Vijaya Rohini Parasuraman, Hsieh-Chih Tsai, Po-Chung Chen, Adhimoorthy Prasannan, Juin-Yih Lai |
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| Rok vydání: | 2019 |
| Předmět: |
Polyethylenimine
Polymers and Plastics Chemistry General Chemical Engineering technology industry and agriculture Nanoparticle 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Controlled release 0104 chemical sciences chemistry.chemical_compound Materials Chemistry Zeta potential Acetone Environmental Chemistry 0210 nano-technology Drug carrier Cytotoxicity Nuclear chemistry Alginic acid |
| Zdroj: | Reactive and Functional Polymers. 145:104238 |
| ISSN: | 1381-5148 |
| DOI: | 10.1016/j.reactfunctpolym.2019.03.006 |
| Popis: | Self-assembly of nanoparticles (NP) with suitable sizes and surface charge to satisfy the functions of an efficient drug carrier plays a vital role in cancer therapy. Hence, the present study focused to prepare polymeric nanoparticles consisting of branched polyethylenimine (PEI), alginic acid (ALG) and poly(cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK), which were used to deliver an anticancer drug 5-fluorouracil (5-FU) to cancer cells. The size of the polymeric nanoparticles, measuring ~11 nm was confirmed by DLS and TEM analysis were revealed nanoparticles formation. Positive zeta potential of nanoparticles indicated that PEI forms the outer layer and the self-assembled of PEI, ALG and PCADK nanoparticles exhibited the fluorescence property. 1H NMR and FTIR analysis confirmed the structural interactions between the PEI, ALG and PCADK during the formation of the nanoparticles. Drug release profiles across a 104-hour period comprising of both burst releases and sustained releases that resulted in 82%, 77% and 52% release of 5-FU at pH 5, 6.8 and 7.4 respectively. Cytotoxicity assay results showed that the 5-FU loaded nanoparticles exhibited greater cytotoxicity against WiDr colorectal adenocarcinoma cell lines compared to free 5-FU due to better adhesion to the cancer cells. |
| Databáze: | OpenAIRE |
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