CDNA AND GENOMIC CLONES FOR HUMAN PLATELET GLYCOPROTEIN lib (GPIIb)
| Autor: | Robin Eisman, Eiias Schwartz, Saul Surrev, Randy A Heidenreich, Paul LaRocco, Joel S. Bennett, Mortimer Poncz |
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| Rok vydání: | 1987 |
| Předmět: | |
| Zdroj: | Pediatric Research. 21:290A-290A |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-198704010-00739 |
| Popis: | The platelet GPIIb-IIIa heterodimer complex is essential for platelet aggregation and is a member of a family of adhesive protein receptors. GPIIb, the larger component of this complex, is composed of two disulfide-linked subunits: GPIIbα and GPHbβ. A cDNA expression library was constructed using mRNA from a human erythroleukemia (HEL) cell line expressing GPIIb-IIIa in λgt11, and the library was screened using a polyclonal antibody against GPIIb. Positive clones were isolated and a 3.3 kb cDNA clone was shown to contain DNA sequence information for the amino acids at the N-termini of both GPIIbα and GPIIbβ. The IIbα sequence begins 30 bp downstream from the 5′-end of the clone, while the IIbβ starts 700 bp upstream from the poly-A tail at the 3′-end of the clone. Northern blot analysis indicates a GPIIb mRNA of 4.1 kb. Analysis of the DNA sequence provides the first complete amino acid sequence for GPIIbα and GPIIbβ, including hydrophobic domains, glycosylation sites, and proline-rich areas. A 16.3 kb human genomic clone has been isolated; exons span at least 12 kb of this clone. Current studies are defining the intron-exon organization of the gene. These findings will allow a direct approach to analysis of the genetic defect(s) causing thrombasthenia, and may lead to means for prenatal diagnosis of this platelet defect. |
| Databáze: | OpenAIRE |
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