Bioprospecting Thermophilic Microorganisms from Icelandic Hot Springs for Hydrogen and Ethanol Production

Autor: Johann Orlygsson, Jaakko A. Puhakka, Steinar R. Beck, Perttu Koskinen, Anna H. Kaksonen, Chiu-Yue Lin, Katariina E.S. Tolvanen, Chyi-How Lay
Rok vydání: 2007
Předmět:
Zdroj: Energy & Fuels. 22:134-140
ISSN: 1520-5029
0887-0624
DOI: 10.1021/ef700275w
Popis: Fermentations can be used to produce sustainable energy carriers, such as hydrogen and ethanol (EtOH), from biomass or organic waste materials. The aim of this research was to prospect efficient H2- and EtOH-producing thermophilic microorganisms derived from hot spring environments in Iceland. Hydrogen- and EtOH-producing enrichment cultures were obtained from various hot spring samples over a temperature range of 50–78 °C. The temperature dependencies for the most promising enrichments were determined with a temperature-gradient incubator. One of the enrichments (33HL) produced 2.10 mol of H2/mol of glucose at 59 °C. Another enrichment (9HG), dominated by bacteria closely affiliated with Thermoanaerobacter thermohydrosulfuricus, produced 0.68 mol of H2/mol of glucose, and 1.21 mol of EtOH/mol of glucose at 78 °C. Hydrogen and EtOH production by 9HG was characterized further in a continuous-flow bioreactor at 74 °C. The highest H2 and EtOH yields of 9HG were obtained at pH 6.8 ± 0.3. Lactate production de...
Databáze: OpenAIRE