Characterization of the asialoglycoprotein receptor in a continuous hepatoma line

Autor:	Alan L. Schwartz, Harvey F. Lodish, Barbara B. Knowles, S E Fridovich
Rok vydání:	1981
Předmět:	education.field_of_study Chemistry Cell Kinetics Population Asialoglycoprotein Cell Biology Ligand (biochemistry) Biochemistry Molecular biology medicine.anatomical_structure Cell culture medicine Asialoglycoprotein receptor Receptor education Molecular Biology
Zdroj:	Journal of Biological Chemistry. 256:8878-8881
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(19)52477-2
Popis:	The asialoglycoprotein receptor has been identified on a continuous human hepatoma cell line, HepG2. This receptor requires Ca2+ for ligand binding and is specific for asialoglycoprotein. There are approximately 150,000 ligand molecules bound/cell at 4 degrees C. These receptors represent a homogeneous population of high affinity binding sites with Kd = 7 X 10(-9) M. From the rate of 125I-ASOR binding at 4 degrees C, kon was 0.95 X 10(6) M-1 min-1. Uptake of 125I-ASOR at 37 degrees C was approximately 0.02 pmol/min/10(6) cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::904efcf009ad14f2390430d3efedfda3 https://doi.org/10.1016/s0021-9258(19)52477-2 Zobrazit plný text záznamu