Coupled chemical reactions in dynamic nanometric confinement: VII. Biosensors based on swift heavy ion tracks with membranes
| Autor: | D. Fink, G. Muñoz H., H. Garcia-Arrelano, L. Alfonta, J. Vacik, A. Kiv, V. Hnatowicz |
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| Rok vydání: | 2017 |
| Předmět: |
Nuclear and High Energy Physics
Analyte Radiation biology Chemistry Ion track 010401 analytical chemistry Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Chemical reaction 0104 chemical sciences Nanopore Membrane Swift heavy ion Chemical engineering biology.protein General Materials Science Glucose oxidase 0210 nano-technology Biosensor |
| Zdroj: | Radiation Effects and Defects in Solids. 172:159-173 |
| ISSN: | 1029-4953 1042-0150 |
| DOI: | 10.1080/10420150.2017.1290633 |
| Popis: | In previous papers it was shown that the coupling of the two chemical reactions: {NaOH etchant – PET polymer} and {NaOH etchant – AgNO3 solution} within the dynamic confinement of etched swift heavy ion tracks eventually leads to the formation of tiny Ag2O membranes within these nanopores, thus separating the latter ones into two adjacent segments. It is shown here that the deposition of enzymes in these two segments transforms these structures into biosensors. In our earlier developed sensors with transparent etched ion tracks, we frequently used glucose oxidase as enzyme and glucose as analyte. In these cases, the enzymatic reaction within the tracks leads to a change in the pH value of the confined solution and hence also in the track conductivity, so these structures can be used for biosensing. When applying, for easy comparison, the same enzyme/analyte combination to the segmented sensor arrangement presented here, we find a striking improvement in detection sensitivity which points at a diff... |
| Databáze: | OpenAIRE |
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