| Popis: |
The DNA-dependent RNA polymerases of pro- and eukaryotes are enzmyes of complex structures consisting of two large and one to ten small molecular weight polypeptides and interacting with an as yet undetermined number of regulatory proteins. The two large subunits appear to harbor the binding sites to DNA and most if not all catalytic functions. We have cloned the gene coding for the 140 kd subunit of RNA polymerase II of Drosophila, and we have identified the gene through a fusion protein produced in an expression vector which, on peptide mapping, yielded identical spots to the 140 kd subunit of purified RNA polymerase II. |
