Immunological Technique for Measuring Insect Contamination of Grain
| Autor: | W. Burkholder, G. B. Kitto, F. A. Quinn |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Larva
Ecology medicine.diagnostic_test Weevil media_common.quotation_subject fungi food and beverages General Medicine Insect Contamination Biology biology.organism_classification Agronomy Biochemistry Polyclonal antibodies Insect Science Immunoassay Myosin biology.protein medicine PEST analysis media_common |
| Zdroj: | Journal of Economic Entomology. 85:1463-1470 |
| ISSN: | 1938-291X 0022-0493 |
| DOI: | 10.1093/jee/85.4.1463 |
| Popis: | An immunological assay to measure insect contamination of grain was established. Polyclonal antibodies were prepared against the insect muscle protein myosin from crickets. These antibodies were used to develop an enzyme-linked immunosorbent assay (ELISA). The ELISA can sensitively measure low nanogram quantities of insect myosin, and can detect a wide variety of common insect pests of stored grain. The assay can quantitatively detect as little as one granary weevil per 50 g of grain and can detect adult, pupal, and larval life stages, but not eggs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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