PRC2 recruitment and H3K27me3 deposition at FLC require FCA binding of COOLAIR
| Autor: | Chao Xu, Han Zheng, Fei Zhang, Xiaoru Ji, Shiliang Wang, Yongke Tian, Yuehui He, Yong Ding |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Regulation of gene expression 0303 health sciences Multidisciplinary biology Chemistry Protein subunit RNA-binding protein macromolecular substances Plasma protein binding biology.organism_classification 01 natural sciences Cell biology Antisense RNA 03 medical and health sciences Transcription (biology) Arabidopsis Flowering Locus C 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Science Advances. 5 |
| ISSN: | 2375-2548 |
| Popis: | The cold-induced antisense transcript COOLAIR represses FLOWERING LOCUS C (FLC) transcription with increased H3K27me3 and decreased H3K36me3 levels in response to cold temperatures. However, the molecular connection between COOLAIR and histone modification factors in the absence of cold treatment remains unclear. We report that the RNA binding protein FCA interacts with the PRC2 subunit CURLY LEAF (CLF) and binds nascent COOLAIR transcripts to allow deposition of H3K27me3 at FLC. Loss of COOLAIR function results in a reduction in FCA and CLF enrichment, which, in turn, decreases H3K27me3 levels at FLC. The Arabidopsis protein phosphatase SSU72 physically interacts with the RRM1 motif of FCA to antagonize FCA binding with COOLAIR. Mutations in SSU72 caused early flowering, reduced FLC transcription, increased CLF enrichment and H3K27me3, and enhanced affinity between FCA and COOLAIR. Our results suggest that FCA binding of COOLAIR and SSU72 is critical for PRC2 enrichment and H3K27me3 deposition in Arabidopsis. |
| Databáze: | OpenAIRE |
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