Determination of β-Agonist Residues in Bovine Urine Using Liquid Chromatography-Tandem Mass Spectrometry
| Autor: | James D MacNeil, Stephen C. Lee, Leslie C Dickson, Adrian C E Fesser |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pharmacology
Chromatography Mabuterol Atmospheric-pressure chemical ionization Mass spectrometry Analytical Chemistry Ractopamine chemistry.chemical_compound chemistry Cimaterol Liquid chromatography–mass spectrometry Environmental Chemistry Gas chromatography–mass spectrometry Agronomy and Crop Science Quadrupole mass analyzer Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 88:46-56 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/88.1.46 |
| Popis: | A multiresidue method was developed and validated to screen bovine urine samples for 10 β-2-adrenergic agonistic drugs—brombuterol, cimaterol, clenbuterol, clenpenterol, isoxsuprine, mabuterol, ractopamine, ritodrine, salbutamol, and tulobuterol—at the 2 μg/L level. The method is also quantitative in the range of 1 to 4 μg/L for all analytes except salbutamol. The procedure uses enzymatic digestion, liquid–liquid extraction, and cleanup on solid-phase extraction columns, followed by detection using a liquid chromatograph-tandem quadrupole mass spectrometer operated in the positive-ion atmospheric pressure chemical ionization multiple-reaction monitoring mode. Method validation included assessment of recoveries, repeatabilities, linearity of responses, decision limits, and detection capabilities. Overall average recoveries ranged from 70–91%; recoveries were generally lower for salbutamol. The decision limits ranged from 0.4–1.0 μg/L, and detection capabilities from 0.6–1.7 μg/L. |
| Databáze: | OpenAIRE |
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