| Autor: | Navaratne B. Eriyagama, R. P. V. J. Rajapakse, N.A.N.D. Perera, S. N. Arseculeratne, D. N. Atapattu |
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| Rok vydání: | 1999 |
| Předmět: |
Antiserum
Differential centrifugation Rhinosporidium seeberi Chromatography Veterinary (miscellaneous) Biology biology.organism_classification Applied Microbiology and Biotechnology Microbiology Endospore Staining chemistry.chemical_compound chemistry Agarose Centrifugation Agronomy and Crop Science Percoll |
| Zdroj: | Mycopathologia. 145:113-119 |
| ISSN: | 0301-486X |
| Popis: | Human rhinosporidial tissue was used as the source of the various developmental stages of Rhinosporidium seeberi - endospores with electron dense bodies, juvenile, and immature sporangia. After homogenisation in phosphate buffered saline (PBS) and removal of tissue fragments by centrifugation, the rhinosporidial bodies were isolated on centrifuged Percoll columns with gradients of densities or on triple-layered columns of varying density. The separated bands, after repeated washing in PBS gave bodies free from human tissue as shown on Leishman and PAS staining and indirect immunofluorescence with rabbit and human patients' anti-rhinosporidial sera. Sonicates of these bodies were tested on agarose gel for precipitation with antisera, and on SDS-PAG electrophoresis and Coomassie Blue staining. Percoll columns were shown to be capable of isolating these stages of R. seeberi, free from human tissue and contaminating bacteria. |
| Databáze: | OpenAIRE |
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