Lipid analysis of Greek broad bean oil: Preparation of liposomes and physicochemical characterization

Autor: Aristidis Georgopoulos, Sophia Hatziantoniou, Agni Paradissis, Costas Demetzos
Rok vydání: 2005
Předmět:
Zdroj: European Journal of Lipid Science and Technology. 107:799-804
ISSN: 1438-9312
1438-7697
Popis: Liposomes were prepared from the isolated phospholipids of mature broad bean [Vicia faba L. (syn. Fabae calabaricae)] oil and their physical properties were studied. The method of preparation was the hydration of the thin lipid film, while the probe sonication methodology was used for reducing the size of the vesicles. The seeds of the broad bean were collected in two different periods of maturity and extracted by the Bligh-Dyer method, and the lipid classes were studied by HPTLC/FID. The oils were found to be rich in polar lipids (63.1% and 60.2% of total lipids) and low in neutral lipids (36.9% and 39.8% of total lipids) for the immature and mature seed oils, respectively. The neutral lipid fraction consisted mainly of triacylglycerides (34.2% and 32.3%) whereas the polar lipids mainly consisted of phospholipids (60.2% and 54.2%) for the mature and immature seed oils, respectively. Sphingolipids (8.9%) were identified only in the immature seed oil. The overall goal of this study was the preparation of a new liposomal formulation with physicochemical properties such as unique lipid composition, size and ζ-potential, which are important factors influencing drug delivery to the target tissues.
Databáze: OpenAIRE
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