Effects of Aerobic Growth on the Fatty Acid and Hydrocarbon Compositions of Geobacter bemidjiensis BemT
| Autor: | Takako Matsushita, Mikako Hashimoto, Satoru Shimizu, Akio Ueno, Hidetoshi Okuyama, Takumi Adachi, Kiyohito Yoshida |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Geobacter bemidjiensis biology General Chemical Engineering 030106 microbiology Fatty acid General Medicine General Chemistry biology.organism_classification 03 medical and health sciences chemistry.chemical_compound Hydrocarbon chemistry Biochemistry Anaerobic exercise Fatty acid synthesis Fatty acid methyl ester Geobacter Polyunsaturated fatty acid |
| Zdroj: | Journal of Oleo Science. 66:93-101 |
| ISSN: | 1347-3352 1345-8957 |
| DOI: | 10.5650/jos.ess16122 |
| Popis: | Geobacter spp., regarded as strict anaerobes, have been reported to grow under aerobic conditions. To elucidate the role of fatty acids in aerobiosis of Geobacter spp., we studied the effect of aerobiosis on fatty acid composition and turnover in G. bemidjiensis BemT. G. bemidjiensis BemT was grown under the following different culture conditions: anaerobic culture for 4 days (type 1) and type 1 culture followed by 2-day anaerobic (type 2) or aerobic culture (anaerobic-to-aerobic shift; type 3). The mean cell weight of the type 3 culture was approximately 2.5-fold greater than that of type 1 and 2 cultures. The fatty acid methyl ester and hydrocarbon fraction contained hexadecanoic (16:0), 9-cis-hexadecenoic [16:1(9c)], tetradecanoic (14:0), tetradecenoic [14:1(7c)] acids, hentriacontanonaene, and hopanoids, but not long-chain polyunsaturated fatty acids. The type 3 culture contained higher levels of 14:0 and 14:1(7c) and lower levels of 16:0 and 16:1(9c) compared with type 1 and 2 cultures. The weight ratio of extracted lipid per dry cell was lower in the type 3 culture than in the type 1 and 2 cultures. We concluded that anaerobically-grown G. bemidjiensis BemT followed by aerobiosis were enhanced in growth, fatty acid turnover, and de novo fatty acid synthesis. |
| Databáze: | OpenAIRE |
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