Reduced sodium pump activity in inositol-deficient HL-60 cells

Autor:	Alison J. Buckenham, Julien Allard, David P.W. Lennon, Mark R. Greenwood, John N. Hawthorne
Rok vydání:	1993
Předmět:	chemistry.chemical_classification Chemistry Diaphragm pump Cell Biology chemistry.chemical_compound Enzyme Mechanism of action Biochemistry medicine Staurosporine Inositol medicine.symptom Protein kinase A Molecular Biology Protein kinase C medicine.drug Diacylglycerol kinase
Zdroj:	Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1220:66-68
ISSN:	0167-4889
DOI:	10.1016/0167-4889(93)90098-a
Popis:	HL-60 cells were cultured in normal and inositol-deficient media. The inositol-deficient cells showed reduced sodium pump activity, as measured by ouabain-sensitive 86Rb+ uptake. The protein kinase C inhibitors staurosporine and H7 did not affect uptake in either normal or inositol-deficient cells. However, U73122, a steroidal inhibitor of phosphoinositidase C, inhibited uptake in both types of cells. Activators of protein kinase C had no effect on Rb+ entry. The inositol deficiency is not considered to affect the sodium pump by a mechanism involving diacylglycerol and protein kinase C.
Databáze:	OpenAIRE
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