Influence of the metabolic state on the tolerance ofPichia kudriavzeviito heavy metals
| Autor: | Cristina Ferreira Silva, Manuela D. Machado, Vanessa Alvarenga Mesquita, Eduardo V. Soares |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Metabolic state Cadmium biology chemistry.chemical_element Heavy metals General Medicine Zinc 010501 environmental sciences biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Yeast 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry Toxicity Sodium azide 0105 earth and related environmental sciences Pichia |
| Zdroj: | Journal of Basic Microbiology. 56:1244-1251 |
| ISSN: | 0233-111X |
| DOI: | 10.1002/jobm.201600232 |
| Popis: | This work aims to examine the influence of the metabolic state of the yeast Pichia kudriavzevii on the susceptibility to a metals mixture (5 mg L-1 Cd, 10 mg L-1 Pb, and 5 mg L-1 Zn). Cells exposed to the metals mixture in the presence of 25 mmol L-1 glucose displayed a higher loss of membrane integrity and proliferation capacity, compared to cells incubated in the absence of glucose. The analysis of the effect of individual metals revealed that glucose increased the toxic effect of Cd marginally, and of Pb significantly. The increased susceptibility to heavy metals due to glucose was attenuated in the simultaneous presence of a mitochondrial respiration inhibitor such as sodium azide (NaN3 ). ATP-depleted yeast cells, resulting from treatment with the non-metabolizable glucose analogue 2-deoxy-d-glucose, showed an increased susceptibility to heavy metals mixture. Pre-incubation of yeast cells with 1 or 1.5 mmol L-1 Ca2+ reduced significantly (P |
| Databáze: | OpenAIRE |
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