Laser phase plate for transmission electron microscopy
| Autor: | Osip Schwartz, Holger Müller, Carter Turnbaugh, Jeremy J. Axelrod, Sara L. Campbell, Robert M. Glaeser |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Materials science business.industry Phase-contrast imaging Phase (waves) Cell Biology Electron Laser Biochemistry law.invention 03 medical and health sciences Amorphous carbon Transmission electron microscopy law Microscopy Optoelectronics business Molecular Biology Electron scattering 030304 developmental biology Biotechnology |
| Zdroj: | Nature Methods. 16:1016-1020 |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/s41592-019-0552-2 |
| Popis: | Transmission electron microscopy (TEM) of rapidly frozen biological specimens, or cryo-EM, would benefit from the development of a phase plate for in-focus phase contrast imaging. Several types of phase plates have been investigated, but rapid electrostatic charging of all such devices has hindered these efforts. Here, we demonstrate electron phase manipulation with a high-intensity continuous-wave laser beam, and use it as a phase plate for TEM. We demonstrate the laser phase plate by imaging an amorphous carbon film. The laser phase plate provides a stable and tunable phase shift without electrostatic charging or unwanted electron scattering. These results suggest the possibility for dose-efficient imaging of unstained biological macromolecules and cells. |
| Databáze: | OpenAIRE |
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