| Autor: | K. Tran Thanh Van, Duong Tan Nhut, Nguyen Tri Minh, Bui Van Le, Michio Tanaka, Seiichi Fukai, Jaime Teixeira de Silva |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
1-Naphthaleneacetic acid Chromatography Somatic embryogenesis Lilium Physiology Plant Science Biology biology.organism_classification Plantlet chemistry.chemical_compound Murashige and Skoog medium chemistry Micropropagation Auxin Botany Agronomy and Crop Science Explant culture |
| Zdroj: | Plant Growth Regulation. 37:193-198 |
| ISSN: | 0167-6903 |
| DOI: | 10.1023/a:1020532511081 |
| Popis: | Somatic embryogenesis was achieved directly from pseudo-bulblettransverse thin cell layers (tTCL) of Lilium longiflorum.Embryo-like structures (globular embryos) were obtained from different sizeexplants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog,1962 (MS) medium containing 5.4 μM naphthalene acetic acid(NAA)and 1.1 μM thidiazuron (TDZ). The embryo-like structures werethen isolated and mass proliferated on MS medium, containing 5.4μM NAA and 0.4 μM TDZ, every 45 days. A0.8–1.0 mm thick explant was shown to be optimal forobtaining the highest number of embryo-like structures. For plant regenerationthese structures were transferred to hormone-free MS medium with 30g/l sucrose. All of these structures formedplantlets after 90 days culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink