Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia
| Autor: | Patricio Rossi, Angela Meza, Yara Prades, María Luisa Garmendia, Luis F. Avendaño, Mauricio Ruiz, Luis Lizama, Rolando Pizarro, Carmen Larrañaga, Vivian Luchsinger |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.diagnostic_test 030106 microbiology Biology Immunofluorescence medicine.disease Virology law.invention 03 medical and health sciences Pneumonia Infectious Diseases Real-time polymerase chain reaction Community-acquired pneumonia law Nasopharyngeal aspirate medicine Respiratory system Viral shedding Polymerase chain reaction |
| Zdroj: | Journal of Medical Virology. 88:1173-1179 |
| ISSN: | 0146-6615 |
| DOI: | 10.1002/jmv.24463 |
| Popis: | Community-acquired pneumonia (CAP) is the third cause of death worldwide. Viruses are frequently detected in adult CAP. Highly sensitive diagnostic techniques should be used due to poor viral shedding. Different sampling methods can affect viral detection, being necessary to establish the optimal type of sample for identifying respiratory viruses in adults. The detection rates of respiratory viruses by Luminex xTAG® RVP fast assay, real time RT-PCR (rtRT-PCR) (Sacace®), and immunofluorescence assay (IFA) in adult CAP were performed in nasopharyngeal swabs (NPS) and aspirates (NPA) from 179 hospitalized adults. Positivity was 47.5% for Luminex®, 42.5% for rtRT-PCR (P = 0.3), and 2.7% for IFA (2.7%) (P |
| Databáze: | OpenAIRE |
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