Use of HgCl2 to investigate dynamic phenomena in yeast cytoplasm

Autor:	W. Mailinger, Uwe Theobald, Manfred Rizzi
Rok vydání:	1994
Předmět:	biology Saccharomyces cerevisiae Metabolism biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Yeast Cell membrane Cell activity medicine.anatomical_structure Cytoplasm medicine Biophysics Differential extraction Yeast metabolism
Zdroj:	Biotechnology Techniques. 8:723-728
ISSN:	1573-6784 0951-208X
DOI:	10.1007/bf00151476
Popis:	To investigate dynamic phenomena in yeast cytoplasm a method was developed that guarantees immediate inactivation of cell activity. Differential extraction procedure gains porosity of the outer cell membrane exclusively. Application of the method during transition from glucose limitation to glucose excess in Saccharomyces cerevisiae illustrates the dynamic behaviour of yeast metabolism and emphasized the necessity of intracellular compartmentation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8fa03122f4dae310a84989ab9bca6beb https://doi.org/10.1007/bf00151476 Zobrazit plný text záznamu Full text from SpringerLink