Identification of Heparin-binding Proteins on the Cell Surface of Cryptococcus neoformans
| Autor: | Ayano Kikuchi, Kohei Kawamura, Tomoe Ichikawa, Yuki Ogasawara, Yo-ichi Ishida, Reiko Ikeda, Yu-ki Tsukiji |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cryptococcus neoformans Gel electrophoresis biology Chemistry 030106 microbiology Cell Virulence Heparin biology.organism_classification Microbiology DNA-binding protein 03 medical and health sciences 030104 developmental biology Infectious Diseases medicine.anatomical_structure Biochemistry medicine Surface plasmon resonance Transaldolase medicine.drug |
| Zdroj: | Medical Mycology Journal. 59:E47-E52 |
| ISSN: | 2186-165X 2185-6486 |
| DOI: | 10.3314/mmj.18-00001 |
| Popis: | Interactions between virulence factors of pathogens and host responses play an important role in the establishment of infection by microbes. We focused on interactions between Cryptococcus neoformans proteins and heparin, which is abundant on host epithelial cells. Surface proteins were extracted and analyzed. Fractions from anion-exchange column chromatography interacted with heparin in surface plasmon resonance analyses. Heparin-binding proteins were purified and then separated by gel electrophoresis; and were identified as transaldolase, glutathione-disulfide reductase, and glyoxal oxidase. These results imply that multifunctional molecules on C. neoformans cells, such as those involved in heparin binding, may play roles in adhesion that trigger responses in the host. |
| Databáze: | OpenAIRE |
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