Rapid Voltammetric Diagnosis of Escherichia Coli Contamination in Non-Treated Human Blood Plasma of Healthy and Sepsis-Infected Patients
| Autor: | Huck Jun Hong, Jeongha Kim, Kyung Lee, Yeoung Chan Kim, Suw Young Ly |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Detection limit
Working electrode Chromatography Stripping (chemistry) Chemistry 010401 analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease_cause 01 natural sciences Reference electrode 0104 chemical sciences Blood plasma medicine Electrical and Electronic Engineering Cyclic voltammetry 0210 nano-technology Instrumentation Escherichia coli Voltammetry |
| Zdroj: | IEEE Sensors Journal. 18:2200-2205 |
| ISSN: | 2379-9153 1530-437X |
| DOI: | 10.1109/jsen.2018.2792840 |
| Popis: | Escherichia coli ( E. coli ) contamination, a critical indication of sepsis-infection, was detected rapidly in real-time using cyclic voltammetry and square wave stripping voltammetry. Mercury-immobilized carbon nanotube paste working electrode and cost-efficient pencil-rod graphite counter and reference electrodes were used and compared with common-type metal and catalytic probes. Electrolyte solutions that were directly utilized included purified seawater, non-treated healthy human blood plasma, and sepsis-infected blood plasma, where cyclic reaction potential was obtained with +0.2-V oxidation and anodic stripping potential of 0.0–4.0-mL E. coli . The analytical interference effects for in vivo trace metals and other microorganisms were determined. Under optimized stripping conditions, a statistic SW detection limit of 0.08 mg L−1 E. coli was obtained. Diagnostic application was successfully performed on sepsis-infected blood plasma, wherein peak current trend and final para-strength were shown to be in accordance with the linear working-range attained. These methodologies and results are applicable for direct in vitro and in vivo real-time detection of E. coli with optimal cost- and time-efficiency. |
| Databáze: | OpenAIRE |
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