Rabbit whey acidic protein gene upstream region controls high-level expression of bovine growth hormone in the mammary gland of transgenic mice
| Autor: | Marie-Louise Fontaine, Louis-Marie Houdebine, M. Massoud, Dominique Thépot, Guy Kann, Marie-Georges Stinnakre, Eve Devinoy |
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| Rok vydání: | 1995 |
| Předmět: |
Genetically modified mouse
0303 health sciences medicine.medical_specialty biology Transgene 030302 biochemistry & molecular biology Mammary gland Cell Biology Chimeric gene Molecular biology 03 medical and health sciences Endocrinology medicine.anatomical_structure Internal medicine Lactation Gene expression Genetics medicine biology.protein Bovine somatotropin Whey Acidic Protein 030304 developmental biology Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 42:261-267 |
| ISSN: | 1040-452X |
| DOI: | 10.1002/mrd.1080420302 |
| Popis: | Transgenic mice were produced which secreted high levels of bGH into milk. The 6.3-kb upstream region of the rabbit whey acidic protein (rWAP) gene was linked to the structural part of the bovine growth hormone (bGH) gene, and the chimeric gene was introduced into mouse oocytes. bGH was detected by radioimmunoassay in the milk of all resulting transgenic mice. bGH concentrations in milk varied from line to line, from 1.0–16 mg/ml. This expression was not correlated to the number of transgene copies. In all lines studied, the mammary gland was the major organ expressing bGH mRNA during lactation. bGH mRNA concentrations were barely detectable in the mammary gland of cyclic females; they increased during pregnancy. These results show that the upstream region of the rWAP gene harbors powerful regulatory elements which target high levels of bGH transgene expression to the mammary gland of lactating transgenic mice. © 1995 wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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