Optimisation of the critical medium components for better growth of Picochlorum sp. and the role of stressful environments for higher lipid production

Autor: Haifa Chtourou, Dhouha Daassi, Ines Dahmen, Slim Abdelkafi, Fatma Karray, Abdelhafidh Dhouib, Ahlem Jebali, Ilem Hassairi, Sami Sayadi
Rok vydání: 2013
Předmět:
Zdroj: Journal of the Science of Food and Agriculture. 94:1628-1638
ISSN: 0022-5142
Popis: showed that the main fatty acids were palmitic acid (29%), linolenic acid (26.5%), linoleic acid (23.5%), hexadecatrienoic acid (11%) and hexadecadienoic acid (8.5%). As it is known that culture conditions greatly influence the composition of microalgae, the experiments were designed to optimise the composition of the medium in order to increase Picochlorum sp. growth from OD680nm = 0.53 to OD680nm = 2.2 and lipid accumulation from 163gkg −1 to 190gkg −1 . The highest lipid contents of 570 and 585gkg −1 were achieved under phosphate starvation and sodium carbonate supplementation, respectively. Under these conditions, the fatty acid profile is dominated by mono-unsaturated and polyunsaturated acids, and is therefore suitable for aqua-culture feeding. However, under high salinity, growth and lipid synthesis are inhibited, and the fatty acids are saturate, and the product is therefore suitable for biodiesel. CONCLUSION:Thishighlipidcontentrichinessentialfattyacids,omega-6andomega-3,endorsesthiswildstrainofPicochlorum sp. as a promising feedstock for aqua-culture and human nutrition or for the production of biodiesel. c � 2013 Society of Chemical Industry
Databáze: OpenAIRE