Endosidin20 Targets the Cellulose Synthase Catalytic Domain to Inhibit Cellulose Biosynthesis
| Autor: | Chunhua Zhang, Lei Huang, Weiwei Zhang, Mingji Dai, Xiaohui Li, Robert E. McEwan, Nana Liu, Yong Li, Natasha V. Raikhel, Christopher J. Staiger, Nolan Ung, Glenn R. Hicks, Xianglin Yin, Brian P. Dilkes |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Mutation biology ATP synthase Fluorescence recovery after photobleaching Cell Biology Plant Science biology.organism_classification medicine.disease_cause 01 natural sciences Amino acid 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Protein structure Biochemistry chemistry Arabidopsis medicine biology.protein Arabidopsis thaliana Cellulose 010606 plant biology & botany |
| Zdroj: | The Plant Cell. 32:2141-2157 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.20.00202 |
| Popis: | Plant cellulose is synthesized by rosette-structured cellulose synthase (CESA) complexes (CSCs). Each CSC is composed of multiple subunits of CESAs representing three different isoforms. Individual CESA proteins contain conserved catalytic domains for catalyzing cellulose synthesis, other domains such as plant-conserved sequences, and class-specific regions that are thought to facilitate complex assembly and CSC trafficking. Because of the current lack of atomic-resolution structures for plant CSCs or CESAs, the molecular mechanism through which CESA catalyzes cellulose synthesis and whether its catalytic activity influences efficient CSC transport at the subcellular level remain unknown. Here, by performing chemical genetic analyses, biochemical assays, structural modeling, and molecular docking, we demonstrate that Endosidin20 (ES20) targets the catalytic site of CESA6 in Arabidopsis (Arabidopsis thaliana). Chemical genetic analysis revealed important amino acids that potentially participate in the catalytic activity of plant CESA6, in addition to previously identified conserved motifs across kingdoms. Using high spatiotemporal resolution live cell imaging, we found that inhibiting the catalytic activity of CESA6 by ES20 treatment reduced the efficiency of CSC transport to the plasma membrane. Our results demonstrate that ES20 is a chemical inhibitor of CESA activity and trafficking that represents a powerful tool for studying cellulose synthesis in plants. |
| Databáze: | OpenAIRE |
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