Autor: |
N. V. Yunusova, A. A. Grigor’eva, I. V. Kondakova, A. K. Somov, E. A. Tugutova, G. V. Kakurina, M. N. Stakheeva, S. V. Molchanov, Sergey G Afanas'ev, S. N. Tamkovich, E. L. Choinzonov, L. A. Kolomiets |
Rok vydání: |
2017 |
Předmět: |
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Zdroj: |
AIP Conference Proceedings. |
ISSN: |
0094-243X |
DOI: |
10.1063/1.5001659 |
Popis: |
Exosomes are extracellular membrane structures involved in many physiological and pathological processes including cancerogenesis and metastasis. The purpose of the study was to isolate, identify and analyze the total content of exosomes in biological fluids. The exosomes from the plasma and ascites samples of the patients with ovarian cancer, from the blood plasma of the patients with colorectal and head and neck squamous cell cancer as well as from the blood plasma of healthy donors were characterized using transmission electron microscopy and flow cytometry. The subpopulations of the exosomes in the biological fluids of the patients with different types of cancer were similar, but the protein concentrations of exosomes were different. In this paper we present the methodological approaches allowing us to obtain high quality exosome preparations from biological fluids. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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