Determination of Myofiber Types of Mangalarga Mares and Their Hybrids

Autor:	JM Andrade, Guilherme de Camargo Ferraz, Elis B. Santos, Fhf D′angelis, Antonio de Queiroz-Neto
Rok vydání:	2014
Předmět:	medicine.medical_specialty Adenosine triphosphatase biology Mangalarga General Medicine biology.organism_classification Surgery Medius Animal science medicine Myocyte Donkey medicine.symptom Myofibril Muscle contraction Hybrid
Zdroj:	Equine Veterinary Journal. 46:27-27
ISSN:	0425-1644
DOI:	10.1111/evj.12267_81
Popis:	Introduction There are no published studies that characterize the different myofiber types of Mangalarga (ML) horses and hybrids. This study aimed to characterize and compare muscle fibers of the gluteus medius of ML mares and young and inactive mules resulting from the breeding of these mares with a Brazilian donkey. Methods Percutaneous biopsies were performed in 11 mares and their foals (10 mules), at a depth of 60 mm. Fiber types were determined by comparing identical images using both the staining techniques of myofibrillar adenosine triphosphatase (mATPase) and nicotinamide adenine dinucleotide-tetrazolium reductase (NADH-TR). Stains for mATPase were pre-incubated in acid medium followed by alkaline incubation. The frequency (F%) and relative total area (RTA%) of fiber types I, IIA and IIX were analysed. The cross-sectional area (CSA, μm2) was quantified by the NADH-TR technique. The means were compared using ANOVA followed by Tukey test (P
Databáze:	OpenAIRE
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