GROWTH REQUIREMENTS AND THERMAL RESISTANCE OF FUNGI BELONGING TO THE GENUS BYSSOCHLAMYS

Autor:	J. L. Weihe, W. S. Hatcher, J.F. Folinazzo, L.G. Albrigo, E.C. Hill, D. I. Murdock
Rok vydání:	1979
Předmět:	Byssochlamys Brix biology fungi biology.organism_classification Stain Conidium Horticulture Visual detection Genus Botany Sugar Ascus Food Science
Zdroj:	Journal of Food Science. 44:118-122
ISSN:	1750-3841 0022-1147
DOI:	10.1111/j.1365-2621.1979.tb10021.x
Popis:	Studies have been conducted to determine growth requirements for fungi belonging to the genus Byssochlamys. Incubation temperature significantly affected growth, with visual detection in 2 days at 30° C and 4 days at 20° C. Sugar concentration was important in liquid culture techniques, with no growth observed in 30° Brix apple drink base after 2,640 hr vs growth in 15° Brix apple drink base after 48 hr. Thermal death studies of three Byssochlamys isolates (G-5, G-52, and M-78) demonstrated wide variation in heat resistance with z-values of 7°, 9.5°, and 14°F respectively. Excellent electron micrographs were obtained of conidia, aleuriospores, and ascospores using a JEOL JSM 35 scanning electron microscope. The micrographs also revealed asci with thicker walls in older (90 days vs 30 days) M-78 cultures. A modification of the Shaeffer-Fulton Spore Stain Technique was used to stain the ascospores within an intact ascus.
Databáze:	OpenAIRE
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