Parameters affecting recoveries of viable Staphylococcus aureus bioaerosols in liquid-based samplers
| Autor: | Yu-Ju Horng, Meng-Hsuan Lin, Sheng-Hsiu Huang, Ching-Wen Chang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Fluid Flow and Transfer Processes
Atmospheric Science Cellular membrane Environmental Engineering Chromatography 010504 meteorology & atmospheric sciences Chemistry Mechanical Engineering Indoor bioaerosol Sampling (statistics) 010501 environmental sciences medicine.disease_cause 01 natural sciences Pollution Staphylococcus aureus Propidium monoazide medicine Liquid based 0105 earth and related environmental sciences |
| Zdroj: | Journal of Aerosol Science. 136:82-90 |
| ISSN: | 0021-8502 |
| Popis: | This study assessed the factors affecting the performance of liquid-based samplers to recover viable infectious bioaerosols of Staphylococcus aureus based on cellular membrane integrity. By using propidium monoazide and qPCR, two widely-used samplers (all-glass impinger-30 (AGI-30) and BioSampler) filled with three collection fluids (deionized water (DW), Tween mixture (TM) and phosphate-buffered saline (PBS)), were evaluated at five sampling times (3, 6, 15, 30 and 60 min). Effects of liquid refilling every 15 min during 60-min sampling were also studied. Irrespective of sampler type, PBS presented the lowest cell recovery, statistically different from DW (P S. aureus collection in DW by AGI-30 (P 0.05). In contrast, samplings for 15 min or greater resulted in decreased cell recoveries in PBS or TM by both samplers compared to 3-min sampling. Overall, BioSampler collected viable S. aureus greater than AGI-30 (P S. aureus . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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