| Popis: |
Background: Recent studies have shown that Hiwi has a crucial role in stem cell self-renewal in various organisms and it is also associated with some cancers. In the present study, Hiwi expression was examined in different grades of primary human colorectal cancer (CRC), colorectal cancer stem cells (CRCSCs) and HT-29 CRC cell line. Methods and Results: CRC tissue samples were collected from 20 patients with CRC. Furthermore, HT-29 cell line, CRCSC and 13 normal colorectal tissue samples were prepared. Expression of Hiwi at mRNA and protein levels was determined by Real-time PCR, flow cytometry, Western blot and immunohistochemistry. Overexpression of Hiwi was detected in 40% (3.351 ± 2.94, PHiwi in CRCSCs (5.94 ± 0.05, P < 0.005). Hiwi expression in CRCSC was significantly higher compared to the colorectal cancer tissue and HT-29 cells (pHiwi mRNA level was significantly correlated to tumor grade (p < 0.01) and stage (p < 0.01).Conclusions: HiWi can be considered as an oncogene in progressive cancer and, therefore, it can be a valuable biomarker and target in therapeutic procedures. |
