Effect of Enumeration Method on Bifidobacterium Cell Counts in Commercial Powder Products

Autor:	Keiji Iwatsuki, Tomoko Yaeshima, Fumiaki Abe, Masamichi Muto
Rok vydání:	2010
Předmět:	Bifidobacterium species Plate method biology Chemistry Phosphate buffered saline General Medicine biology.organism_classification Diluent Dilution fluids and secretions Infant formula Enumeration Food science Bifidobacterium
Zdroj:	Bioscience and Microflora. 29:143-148
ISSN:	1349-8355 1342-1441
Popis:	In this study, the effects of enumeration methods on viable bifidobacterial counts in powder products were examined to determine the most appropriate enumeration conditions. The bifidobacterial counts of nine commercial powder products containing Bifidobacterium species were determined by a pour plate method using different diluents including Mitsuoka's buffer and Ringer's solution. Mitsuoka's buffer gave significantly higher bifidobacterial counts than Ringer's solution when used as diluent for eight of nine commercial powder products containing various Bifidobacterium species. The counts obtained with Ringer's solution were on average 51.6% of those obtained using Mitsuoka's buffer. Addition of Tween 80 or phosphate buffer to Ringer's solution increased the bifidobacterial counts. Also, the diluent had a significantly greater impact in the suspension step than in the subsequent dilution step. In conclusion, the diluent has great effect on the enumeration of bifidobacteria when using the plate count method. Also, the composition of diluents influences bifidobacterial counts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8f2a1f63c2da5aa32f321908aa122f5b https://doi.org/10.12938/bifidus.29.143 Zobrazit plný text záznamu