Isolation and Characterization of Mannose-Binding Lectin Gene from Leaves of Allium ascalonicum (Shallot) and its Putative Role in Insect Resistance
| Autor: | Sripriya Rajasekaran, K. Rajendran, Ilamathi Raja, Manoharan Kumariah |
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| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | South Indian Journal of Biological Sciences. 2:245 |
| ISSN: | 2454-4787 2454-8510 |
| DOI: | 10.22205/sijbs/2016/v2/i2/100309 |
| Popis: | Plant lectins are the heterogenous group of glycoproteins extensively studied for their potent insecticidal property against Hemipteran pests. In this present study, the full‐length cDNA of monocot mannose‐binding insecticidal lectin gene was isolated from Allium ascalonicum leaves. The isolated Allium ascalonicum Lectin (AAL) gene was cloned in pGEM‐T vector, sequenced and the sequence was submitted to GenBank (KM096570.1). Sequence analysis revealed a 468 bp open reading frame (ORF) encoding a putative 155 amino acids agglutinin precursor. Multiple sequence alignment and phylogenetic analysis of AAL amino acid with those of 30 other Mannose binding lectin (MBL) sequences in NCBI revealed a high similarity of 85‐95% indicating that AAL is a member of the MBL super family and forms a cluster with other onion lectins. Secondary structure prediction and the homology modeling showed that AAL protein possess predominantly β‐sheets and three potential mannose‐binding motifs consisting of 5 amino acid residues QDNVY like other GNA lectins. The results of the insilico analysis predict that the Allium ascalonicum lectin gene can be another potent insecticidal protein. |
| Databáze: | OpenAIRE |
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