Application of RNA-seq and Bioimaging Methods to Study Microbe–Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression

Autor:	Cristina Isabel Amador, Claus Sternberg, Lars Jelsbak
Rok vydání:	2017
Předmět:	0301 basic medicine Host (biology) 030106 microbiology Biofilm RNA Pathogenic bacteria RNA-Seq Biology medicine.disease_cause In vitro Cell biology 03 medical and health sciences 030104 developmental biology Gene expression medicine Pathogen
Zdroj:	Host-Pathogen Interactions ISBN: 9781493976034
DOI:	10.1007/978-1-4939-7604-1_12
Popis:	Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8f26f4353615a3513d2508dbe6227327 https://doi.org/10.1007/978-1-4939-7604-1_12 Zobrazit plný text záznamu