Centrosome overduplication and mitotic instability in PKD2 transgenic lines
| Autor: | Stéphane Burtey, Marta Riera, Marie Geneviève Mattei, Petra Pennenkamp, Roselyne Rance, Michel Fontes, Bernd Dworniczak, Emilie Ribe, Judith Luciani |
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| Rok vydání: | 2008 |
| Předmět: |
Genetically modified mouse
education.field_of_study urogenital system Cilium Autosomal dominant polycystic kidney disease Cell Biology General Medicine Biology urologic and male genital diseases medicine.disease female genital diseases and pregnancy complications Cell biology Polycystin 2 Centrosome medicine Polycystic kidney disease Centrosome duplication education Mitosis |
| Zdroj: | Cell Biology International. 32:1193-1198 |
| ISSN: | 1065-6995 |
| DOI: | 10.1016/j.cellbi.2008.07.021 |
| Popis: | Polycystin-2 (PC-2), a protein encoded by PKD2 and involved in autosomal dominant polycystic kidney disease (ADPKD), is a non-selective cationic channel recently implicated in the function of primary cilia. We recently constructed a new animal model in the form of a transgenic mouse with a BAC-containing human PKD2 inserted in its genome. Two transgenic mouse lines overexpressing human PKD2 showed mitotic instability. Fibroblasts from these transgenic mouse lines have abnormal chromosomal numbers. These lines also have supernumerary centrosomes. PC-2 overexpression is associated with mitotic instability and centrosome overduplication. PC-2 therefore seems to play a role in centrosome duplication, and this hypothesis is being evaluated in other models. |
| Databáze: | OpenAIRE |
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