In situ cultured preantral follicles is a useful model to evaluate the effect of anticancer drugs on caprine folliculogenesis
| Autor: | Vilceu Bordignon, G.Q. Rodrigues, Ana Paula Ribeiro Rodrigues, Carla Renata Figueiredo Gadelha, Laritza Ferreira de Lima, Simone Vieira Castro, Cláudia Pessoa, Denise Damasceno Guerreiro, José Ricardo de Figueiredo, Adeline de Andrade Carvalho, Cláudio Cabral Campello |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty 030219 obstetrics & reproductive medicine Histology Ovarian Cortex Caspase 3 Biology DNA laddering Oogenesis Andrology 03 medical and health sciences Medical Laboratory Technology 030104 developmental biology 0302 clinical medicine Endocrinology medicine.anatomical_structure Internal medicine medicine DNA fragmentation Doxorubicin Folliculogenesis Anatomy Ovarian follicle Instrumentation medicine.drug |
| Zdroj: | Microscopy Research and Technique. 79:773-781 |
| ISSN: | 1059-910X |
| Popis: | Despite the increase in the incidence of cancer, the number of women who survive cancer treatment is growing. However, one of the principal results of chemotherapy is premature ovarian failure (POF). The aim of this study was to use the in situ culture preantral follicles as an in vitro model to evaluate the toxicity of two anticancer drugs, doxorubicin (DXR) and paclitaxel (PTX), on the integrity and development of ovarian follicles. Fragments of the ovarian cortex of goats were cultured in vitro for 1 or 7 days in α-MEM(+) supplemented with different concentrations of DXR (0.003, 0.03, or 0.3 µg/mL) and PTX (0.001, 0.01, or 0.1 µg/mL). Analyses were performed before and after culture to evaluate tissue integrity by classical histology, apoptosis by TUNEL assay, DNA laddering kit and the detection of activated caspase 3, and DNA damage by the immune detection of phosphorylated histone H2A.x (H2AXph139). Both DXR and PTX reduced the number of morphologically normal primordial and developing follicles. Positive staining for TUNEL and active caspase 3 was detected in all the samples (P |
| Databáze: | OpenAIRE |
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