A Resolution Equation for Electrokinetic Chromatography Based on Electrophoretic Mobilities

Autor:	Edward R. Grover, Michael E. Swartz, Jeffrey R. Mazzeo
Rok vydání:	1995
Předmět:	chemistry.chemical_compound Electrokinetic phenomena Electrophoresis Chromatography chemistry Resolution (mass spectrometry) Benzoin Analytical chemistry Electro-osmosis Electrophoretic mobilities Fundamental Resolution Equation Enantiomer Analytical Chemistry
Zdroj:	Analytical Chemistry. 67:2966-2973
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac00113a035
Popis:	A resolution equation for electrokinetic chromatography (EKC) was developed starting from the resolution equation for electrophoresis. The equation was used to predict the influences of the migration window and partitioning on resolution in EKC. It is theoretically shown that the migration window can have a dramatic effect on resolution in EKC. Using a novel chiral surfactant, the influences of the migration window and partitioning on the separation of benzoin enantiomers were experimentally determined. The results obtained agreed with predictions based on the equation. The ability to obtain very high resolution values by migration window manipulation was demonstrated for the separation of N-methylpseudoephedrine enantiomers (α = 1.3). Specifically, the resolution was 2.4 under conditions of robust electroosmotic flow (EOF) but increased to 11 when conditions of low EOF were employed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8ef359e97ca75f5679bd5db8ab7d84be https://doi.org/10.1021/ac00113a035 Zobrazit plný text záznamu