Genetic analysis of nuclear ribosomal DNA of Lentinula edodes

Autor:	Yoko Ono, Masaki Fukuda, Tadashi Sekiguchi
Rok vydání:	2006
Předmět:	Genetics Lentinula biology Tandem repeat Genetic marker Genotype Restriction fragment length polymorphism biology.organism_classification Ribosomal DNA Molecular biology Genetic analysis Ecology Evolution Behavior and Systematics Dikaryon
Zdroj:	Mycoscience. 47:347-350
ISSN:	1340-3540
Popis:	Genetic analysis of nuclear ribosomal DNA (rDNA) of Lentinula edodes was carried out using rDNA restriction fragment length polymorphisms (RFLPs) as genetic markers. Two compatible monokaryotic strains that differed in the endonuclease digestion patterns of their rDNA were used. The dikaryotic strain established by crossing them produced mixed RFLP patterns. Single-spore isolates derived from the dikaryotic strain showed three types of rDNA RFLP patterns: either one of the two parental types or a mixed type. From the frequency of the mixed type, the recombination value of rDNA tandem repeats was calculated to be 31.4%. Linkage analysis between rDNA and two incompatibility factors (A and B) revealed that rDNA was not linked to either factor. The rDNA genotypes did not affect mycelial growth among the single-spore isolates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8ef0618a2a5695f6057a7804d17631a4 https://doi.org/10.1007/s10267-006-0314-8 Zobrazit plný text záznamu Full text from SpringerLink