Differential α2A- and α2C-adrenoceptor protein expression in presynaptic and postsynaptic density fractions of postmortem human prefrontal cortex
| Autor: | Iria Brocos-Mosquera, Luis F. Callado, Ane M. Gabilondo, Amaia M. Erdozain, J Javier Meana |
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| Rok vydání: | 2018 |
| Předmět: |
Pharmacology
Adrenergic receptor Chemistry Human brain Protein expression 030227 psychiatry Cell biology 03 medical and health sciences Psychiatry and Mental health 0302 clinical medicine α2a adrenoceptor medicine.anatomical_structure Postsynaptic potential medicine Pharmacology (medical) Prefrontal cortex Postsynaptic density Gene 030217 neurology & neurosurgery |
| Zdroj: | Journal of Psychopharmacology. 33:244-249 |
| ISSN: | 1461-7285 0269-8811 |
| DOI: | 10.1177/0269881118798612 |
| Popis: | Background:Three different α2-adrenoceptor (α2-AR) subtypes have been described. The α2A-AR and α2C-AR subtypes are highly expressed in the human prefrontal cortex, where they modulate neurotransmission. However, due to the lack of subtype-selective ligands, the physiological relevance of both subtypes has not been fully resolved.Aims:In this context, the aim of the present study was to characterize the protein expression of both α2-AR subtypes, in different synaptic fractions of postmortem human prefrontal cortex.Methods:A subcellular fractionation of the samples was performed and the protein expression of α2A- and α2C-ARs was measured in presynaptic membranes and postsynaptic density fractions by Western blot.Results:The results revealed that the α2A-AR subtype is mainly located postsynaptically (95±3%) whereas the remaining 5±3% is in the presynapse. Conversely, the α2C-AR subtype showed a similar distribution between pre- and postsynaptic membranes, with a slightly higher percentage present in the presynapse (60±2% vs. 40±2%).Conclusions:These findings could explain some contradictory effects reported for α2-AR agonists and antagonists in the human prefrontal cortex. Furthermore, the present data could contribute to elucidating the therapeutic potential of selectively targeting α2A- or α2C-AR subtypes. |
| Databáze: | OpenAIRE |
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