High-performance liquid chromatographic study of the chlorophyll allomerization reaction

Autor:	Jerry E. Hunt, Peter M. Schaber, Richard Fries, Joseph J. Katz
Rok vydání:	1984
Předmět:	Reaction mechanism Chromatography Organic Chemistry General Medicine Mass spectrometry Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Nucleophile Deuterium Chlorophyll Bacteriochlorophyll
Zdroj:	Journal of Chromatography A. 316:25-41
ISSN:	0021-9673
DOI:	10.1016/s0021-9673(00)96138-7
Popis:	The allomerization of chlorophyll has been investigated by reversed-phase HPLC. Four major product peaks (two sets of doublets) are observed in the HPLC chromatogram. These are shown to be pairs of stereoisomers of two major components. Similar results have been obtained from the allomerization of fully deuterated chlorophyll a. Attempts to characterize the reaction products and to delineate the reaction mechanism were initially studied by the oxidation of chlorophyll in the absence of extraneous nucleophiles. The structure of the reaction products of chlorophyll allomerization were conclusively identified as 10-hydroxychlorophyll and the 10-methoxylactone by co-chromatography, NMR, and 252Cf plasma-desorption mass spectroscopy. Exploratory studies on the allomerization products of bacteriochlorophyll have also been carried out.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8ee0bfda78fa1f08699fda3ffd59c1c5 https://doi.org/10.1016/s0021-9673(00)96138-7 Zobrazit plný text záznamu