Unveiling soil food web links: New PCR assays for detection of prey DNA in the gut of soil arthropod predators
| Autor: | Stefan Scheu, Bernhard Eitzinger, Aleksandra Micic, Michael Traugott, Maximilian Körner |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
0303 health sciences biology Ecology Soil Science 15. Life on land biology.organism_classification 010603 evolutionary biology 01 natural sciences Microbiology Predation law.invention 03 medical and health sciences Isopoda law Soil food web Arthropod Oribatida Polymerase chain reaction 030304 developmental biology Invertebrate Trophic level |
| Zdroj: | Soil Biology and Biochemistry. 57:943-945 |
| ISSN: | 0038-0717 |
| DOI: | 10.1016/j.soilbio.2012.09.001 |
| Popis: | Molecular gut content analysis provides a highly specific and sensitive tool to examine the diet of soil invertebrates. Here, we present new polymerase chain reaction (PCR) assays for the detection of twelve prey taxa common in Central European forest soils. The assays target five species of collembolans as well as dipterans, gamasid and oribatid mites, lithobiid centipedes, spiders, staphylinid beetles and woodlice at the group level, amplifying 123–299 bp long DNA fragments. Cross-reactivity tests against 119 soil invertebrate taxa confirm their specificity. These new PCR assays were found to be highly sensitive, revealing the consumption of five different prey taxa in field-collected centipedes. Thus they provide a ready-to-use approach for unravelling trophic interactions among soil arthropods. |
| Databáze: | OpenAIRE |
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