Characterization of DNA synthesis and chloroplast DNA replication initiation in a Petunia hybrida chloroplast lysate system

Autor:	Jan M. de Haas, J. Nijkamp, Wieger van Brug, Nico Overbeeke, A.J. Kool, H. John
Rok vydání:	1987
Předmět:	DNA clamp biology DNA polymerase DNA polymerase II DNA replication food and beverages General Medicine Molecular biology Replication Initiation Genetics biology.protein Primase DNA polymerase I In vitro recombination
Zdroj:	Current Genetics. 12:377-386
ISSN:	1432-0983 0172-8083
DOI:	10.1007/bf00405760
Popis:	An in vitro chloroplast DNA synthesizing lysate system prepared from purified chloroplasts of Petunia hybrida leaves has been developed. Both co-isolated endogeneous chloroplast (cp)DNA and externally added DNA can be used as DNA templates in the system. The system contains a γ-like DNA polymerase as determined by using DNA polymerase-specific inhibitors and synthetic templates. The molecular weight of this enzyme is about 85 kd. Part of the DNA synthesizing activity is repair synthesis. When a chimaeric plasmid containing a fragment with a potential cpDNA replication origin is used as a template (pPCY62), specific initiation of DNA synthesis is observed on this fragment which strongly suggests that the in vitro chloroplast lysate system is also capable of replication initiation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8ed55a2b233bd20277b5679e2ebd4c07 https://doi.org/10.1007/bf00405760 Zobrazit plný text záznamu Full text from SpringerLink