Organ-based chemo-profiling of Echinops echinatus Roxb. using high-performance thin-layer chromatography (HPTLC) technique

Autor: Yogita Bansal, Nusrat Fayaz Bhatt, Raghbir Chand Gupta
Rok vydání: 2021
Předmět:
Zdroj: JPC – Journal of Planar Chromatography – Modern TLC. 34:173-181
ISSN: 1789-0993
0933-4173
Popis: Plants contain different classes of secondary metabolites that have different levels of solubility in solvents, based on their polarity. The sequential extraction of Echinops echinatus was carried out using three solvent systems, namely water, hydromethanol, and chloroform, where the ratio of hydromethanol is (30:70V/V). The extracts were subjected to a standard phytochemical analysis against some specific marker compounds, which include the class of triterpenoids, phytosterols, and flavonoids. A varied range of phytochemicals were found in the extracts. Betulinic acid, oleanolic acid, and lupeol were found maximum in the hydromethanolic extract of the root samples (13.42 ± 0.01 mg/g, 10.50 ± 0.01 mg/g, and 9.69 ± 0.02 mg/g, respectively). Phytosterols like β-sitosterol, ergosterol, and campesterol were also found maximum in the hydromethanolic extract of the root samples (11.18 ± 0.01 mg/g, 13.12 ± 0.02 mg/g, and 8.83 ± 0.01 mg/g, respectively). Quercetin, caffeic acid, and chlorogenic acid were found maximum in the water extract of the leaf samples (14.00 ± 1.21 mg/g, 14.5 ± 0.24 mg/g, and 19.25 ± 0.01 mg/g). The present investigation on the medicinal plant E. echinatus has proved that the selection of solvent for extraction should be based on the target compounds. As in the present analysis, triterpenoids and phytosterols were found maximum in the hydromethanol and flavonoids were found maximum in the water extract.
Databáze: OpenAIRE
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