Differentiation of retinal organoids from human pluripotent stem cells
| Autor: | Kirstin B. VanderWall, Sailee S. Lavekar, Jason S. Meyer, Kang-Chieh Huang, Clarisse M. Fligor |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Retina Retinal Biology Retinal ganglion Cell biology 03 medical and health sciences chemistry.chemical_compound medicine.anatomical_structure chemistry Retinal ganglion cell medicine Organoid sense organs Stem cell Induced pluripotent stem cell Muller glia 030304 developmental biology |
| Zdroj: | Methods in Cell Biology ISBN: 9780128215319 |
| DOI: | 10.1016/bs.mcb.2020.02.005 |
| Popis: | Human pluripotent stem cells (hPSCs) possess the remarkable ability to differentiate into any cell type of the body, including those of the retina. Through the differentiation of these cells as retinal organoids, it is now possible to model the spatial and temporal development of the human retina using hPSCs, in which retinal progenitor cells produce the entire repertoire of retinal cells, first differentiating into retinal ganglion cells and ending with mature photoreceptors, bipolar cells, and Muller glia. Importantly, retinal organoids self-assemble into laminated structures that recapitulate the layering of the human retina with a retinal ganglion cell layer lining the inner layer and a distinctly separate photoreceptor layer occupying the outer layers. This organoid technology has provided access to human tissue for developmental and disease modeling, as well as translational applications such as high throughput drug screening and cell replacement therapies. However, the differentiation of retinal organoids does require some expertise and multiple strategies produce inconsistent results. Here, we describe in detail a well-established and relatively simple method for the generation of retinal organoids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|