| Autor: | White K, L. Gregory, Thomas G. Rand, Logan A |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pathology
medicine.medical_specialty Lung Toxin Veterinary (miscellaneous) medicine.medical_treatment Stachybotrys chartarum fungi Histology Biology biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Spore Andrology medicine.anatomical_structure In vivo Hemosiderin medicine Agronomy and Crop Science Saline |
| Zdroj: | Mycopathologia. 156:119-131 |
| ISSN: | 0301-486X |
| Popis: | Stachybotrys chartarum is an important toxigenic fungus often associated with chronically wet cellulose-based building materials. The purpose of this study was to evaluate some histological, immunohistochemical and morphometric changes in mouse lung tissues exposed intratracheally to either 50 μl of 1.4 × 106S. chartarum spores (≤35 ng toxin/kg BW), isosatratoxin-F (35ng/kg BW),50 μl of 1.4 × 106Cladosporium cladosporioides spores, or 50 μl saline. Exposure of lung tissues to S. chartarum or C. cladosporioides spores resulted in granuloma formation at the sites of spore impaction. Some of the lung tissues impacted by S. chartarum spores also showed erythrocyte accumulation in the alveolar air space, dilated capillaries engorged with erythrocytes, and hemosiderin accumulation at spore impaction sites, which were features not noted in the C. cladosporioides-spore treated animals. Immunohistochemistry revealed reduced collagen IV distribution in lung granulomas in S. chartarum-treated animals especially at 48 and 72 hr post-exposure compared to that in lungs of mice with C. cladosporioides-spore induced granulomas. Quantitative analysis of pooled S. chartarum and C. cladosporioides spore impacted lungs revealed significant depression (P < 0.05) of alveolar air space from 71.4 ± 6.1 in untreated animals to 56.04 ± 6.1 in the S. chartarum- and 60.24 ± 5.5% in the C. cladosporioides-spore treated animals. It also revealed that alveolus air space in S. chartarum treated animals declined significantly from 63.74 ± 3.1% at12 hr post-exposure to 42.94 ± 7.9% at 72 hr post-exposure and was increased to 54.84 ± 5.2% at 96 hr post-exposure. Alveolus air space in C. cladosporioidestreated animals also decreased significantly from 64.84 ± 7.1% at 12 hr exposure to 54.94 ± 5.4% at 48 hr post-exposure and was increased to 64.64 ± 10.1% at 96 hr post-exposure. It also revealed significant (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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