| Autor: |
B. Sivak, Tibor Hianik, Maja Šnejdárková, Sergey A. Ivanov, M. Rehák, V.I. Passechnik |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Bioelectrochemistry and Bioenergetics. 45:233-237 |
| ISSN: |
0302-4598 |
| DOI: |
10.1016/s0302-4598(98)00104-4 |
| Popis: |
The study of current fluctuations of metal-supported bilayer lipid membranes (s-BLM) modified by glucose oxidase (GOX) is presented. Current fluctuations are expressed as a dependence of spectral density ( P ) on a frequency and as a dependence of P at certain frequency (10, 100 and 1000 Hz) on the concentration of glucose and/or current flowing across a membrane. We have shown that in the frequency range of 10–1000 Hz, the dependence of spectral density on the frequency of fluctuations can be described by the dependence 1/ f α , where α ∼1. This dependence is typical for fluctuations of the current flowing across free-standing BLM observed earlier. The amplitude of P for s-BLM with immobilized GOX was considerably larger than that without GOX. However, the current flowing across s-BLM was higher than that for s-BLM with immobilized GOX. The origin of fluctuations is explained by means of the peculiarities of s-BLM structure and by the influence of conformational changes of GOX on the properties of supported bilayers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect