Electrospray Ionization Mass Spectrometry of Cyclodextrin Complexes of Amino Acids and Peptides
| Autor: | John R. Eyler, Ragulan Ramanathan, Laszlo Prokai, Joseph P. Nawrocki |
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| Rok vydání: | 1996 |
| Předmět: |
Chromatography
Resolution (mass spectrometry) Protein mass spectrometry Chemistry Electrospray ionization Selected reaction monitoring technology industry and agriculture Top-down proteomics Fourier transform ion cyclotron resonance Sample preparation in mass spectrometry carbohydrates (lipids) Matrix-assisted laser desorption/ionization polycyclic compounds lipids (amino acids peptides and proteins) |
| Zdroj: | Proceedings of the Eighth International Symposium on Cyclodextrins ISBN: 9780792340294 |
| DOI: | 10.1007/978-94-011-5448-2_49 |
| Popis: | Appropriate complex-forming side-chains of peptides and proteins can bind cyclodextrins. We have characterized interactions of amino acids with cyclodextrins by electrospray ionization mass spectrometry. The stoichiometry of various α-cyclodextrin/protonated tryptophan complexes sampled from solution to the gas phase by ESI was revealed by taking advantage of the extended mass range and high mass resolution of a Fourier-transform ion cyclotron resonance instrument. Binding of β-cyclodextrin to recombinant human insulin has been demonstrated. |
| Databáze: | OpenAIRE |
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