Agrobacterium-mediated transformation in Citrullus lanatus

Autor:	Jang Ryol Liu, M A Cho, C Y Moon, P S Choi
Rok vydání:	2008
Předmět:	Citrullus lanatus biology Agrobacterium fungi Paromomycin Plant Science Horticulture biology.organism_classification Transformation (genetics) chemistry.chemical_compound Glufosinate chemistry Botany Shoot medicine Southern blot medicine.drug Explant culture
Zdroj:	Biologia plantarum. 52:365-369
ISSN:	1573-8264 0006-3134
DOI:	10.1007/s10535-008-0076-6
Popis:	Agrobacterium tumefaciens-mediated transformation was used to produce transgenic watermelon. Cotyledonary explants of Citrullus lanatus Thumb (cv. Daesan) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing pPTN289 carrying with bar gene and pPTN290 carrying with nptII gene, respectively. There was a significant difference in the transformation frequency between bacteria strains and selective markers. The EHA101/pPTN289 showed higher transformation frequency (1.16 %) than GV3101/pPTN289 (0.33 %) and LBA4404/pPTN289 or /pPTN290 (0 %). The shoots obtained (633 and 57 lines) showed some resistance to glufosinate and paromomycin, respectively. Of them, the β-glucuronidase positive response and PCR products amplified by bar and nptII specific primers showed at least 21 plants resistant to glufosinate and at least 6 plants to paromomycin. Southern blot analysis revealed that the bar gene integrated into genome of transgenic watermelon. Acclimated transgenic watermelons were successfully transplanted in the greenhouse and showed no phenotypic variation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8eaee06f6a3b87a418338865005f7aba https://doi.org/10.1007/s10535-008-0076-6 Zobrazit plný text záznamu Full text from SpringerLink