Development of two spectrophotometric methods for quantification of certain anti-depressant drug in pure, pharmaceutical formulation and application to content uniformity testing
| Autor: | Mostafa A. Marzouq, Walid E. Eltoukhi, Noha N. Atia, Ahmed I. Hassan |
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| Rok vydání: | 2019 |
| Předmět: |
Drug
Detection limit Chromatography Chemistry media_common.quotation_subject 010401 analytical chemistry 02 engineering and technology Pharmaceutical formulation 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry Anti depressant 0210 nano-technology Spectroscopy media_common |
| Zdroj: | Microchemical Journal. 147:1048-1054 |
| ISSN: | 0026-265X |
| Popis: | Two accurate, rapid, simple, robust and extraction-free spectrophotometric techniques were created for assay of Amisulpride (AMS) in pure and tablet preparation. The described methods have been established on the reaction of methanolic solution of AMS with 4-(dimethylamino) benzaldehyde in acidic medium which give yellow product that can be determined at λmax = 400 nm (method-I) and the reaction AMS with tetrachloro-1, 4-benzoquinone (p-Ch) which yields intense blue to purple colored charge transfer complex that can be determined at λmax = 559 nm (method-II). The prescribed assay obeys Beer's law within the range from 20 to 200 and 50–400 micrograms per milliliter for method-I & method-II orderly. Detection limits have been 4.4 & 10.6 μg/mL while quantitation limits have been 13.2 & 32.0 micrograms per milliliter for method-I & method-II orderly. Developed methods were validated according to guidelines of International conference on Harmonization for the detection of cited drug in pure, pharmaceutical preparations and content uniformity testing. |
| Databáze: | OpenAIRE |
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