| Autor: | Jacques Livage, S. Fennouh, Cécile Roux, S. Guyon |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Chromatography
Materials science biology Silica gel General Chemistry Condensed Matter Physics medicine.disease_cause biology.organism_classification Electronic Optical and Magnetic Materials Biomaterials Entrapment chemistry.chemical_compound Chemical engineering chemistry Transmission electron microscopy Silica matrix Materials Chemistry Ceramics and Composites medicine Cellular organization Escherichia coli Bacteria Sol-gel |
| Zdroj: | Journal of Sol-Gel Science and Technology. 19:647-649 |
| ISSN: | 0928-0707 |
| DOI: | 10.1023/a:1008733916175 |
| Popis: | Whole cell bacteria have been entrapped within sol-gel silica matrices in order to perform bio-catalytic experiments. Escherichia coli have been chosen as a model for sol-gel encapsulation. Transmission electron microscopy shows that bacteria are randomly dispersed within the silica matrix and that their cellular organization is preserved. The β-galactosidase activity of entrapped E. coli was studied using p-NPG as a substrate. The formation of p-nitrophenol was followed by optical absorption. These experiments show that E. coli still exhibit noticeable enzymatic activity after encapsulation in wet gels. They follow the well known Michaelis-Menten kinetic law but their activity decreases in dried xerogels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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