Synthesis of HbA and HbF in erythroid colonies cultured from human fetal liver and umbilical cord blood
| Autor: | M. W. Hassan, LL Cederqvist, R. F. Rieder, A Ibrahim |
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| Rok vydání: | 1979 |
| Předmět: | |
| Zdroj: | Blood. 54:1140-1151 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Erythroid colonies were grown in vitro in 0.1-ml plasma clots from 12 to 17 wk human fetal liver tissue obtained from prostaglandin-induced abortuses, and from full-term umbilical cord bloods. Synthesis of α, β, and γ globin chains in the colonies was measured after varying periods of culture and after short-term incubation of the tissue source from which the colonies were derived. In suspensions of fetal liver cells, the s/γ synthesis ratio averaged 0.12 (range 0.10–0.15). In the erythroid colonies grown from the liver tissue, the average s/γ ratio after 5–14 days of culture was 0.16 (range 0.09–0.24). In cord blood suspensions, the average s/γ ratio was 0.60 (range 0.39–0.74), and in the erythroid colonies, after 9–14 days of culture, it was 0.74 (range 0.42–1.17). A tendency towards an increased ratio was noted in the later clot cultures from both sources. The relative synthesis of β and γ globin in erythroid colonies grown in plasma clots from these “fetal” sources reflected the period of human development from which the colony progenitors were obtained. It would therefore seem that the proportion of HbA and HbF synthesized by developing human erythropoietic tissues is already determined by the stage of erythroid maturation of the precursor cells that function as in vitro colony formers. |
| Databáze: | OpenAIRE |
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