Genomic-based microsatellite development for Ternstroemia (Pentaphylacaceae) and transferability to other Ericales

Autor: Hernán Alvarado-Sizzo, Othón Alcántara-Ayala, David Espinosa, Gerardo Rivas, Ken Oyama, Isolda Luna-Vega
Rok vydání: 2023
Předmět:
Zdroj: Molecular Biology Reports. 50:3547-3555
ISSN: 1573-4978
0301-4851
Popis: Background The genus Ternstroemia is associated with the vulnerable tropical montane cloud forest in Mexico and with other relevant vegetation types worldwide. It contains threatened and pharmacologically important species and has taxonomic issues regarding its species limits. This study describes 38 microsatellite markers generated using a genomic-based approach. Methods and results We tested 23 of these markers in a natural population of Ternstroemia lineata. These markers are highly polymorphic (all loci polymorphic with 3–14 alleles per locus and expected heterozygosity between 0.202 and 0.908), most of them (19 out of 23) are in Hardy-Weinberg Equilibrium and free of null alleles (18 out of 23). Also we found no evidence of linkage among them. Finally, we tested the transferability to six other American species of Ternstroemia, two other Pentaphylacaceae species, and four species from different families within the order Ericales. Conclusions These molecular resources are promising tools to investigate genetic diversity loss and as barcodes for ethnopharmacological applications and species delimitation in the family Pentaphylacaceae and some Ericales, among other applications.
Databáze: OpenAIRE
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